ANTIOXIDANT ENZYMES ACTIVITIES AND PROLINE CONTENT IN LEAVES OF SALIX SPECIES GROWN ON FLY ASH DUMPS

Authors

  • C. BABEANU
  • A. M. DODOCIOIU

Abstract

For remediation and re-cultivation of polluted soils, including fly ash dumps, stress-tolerant plants are needed, one of the species used for this purpose being the willow tree. A first response of the plant to different types of stress is the activation of the enzymatic and non-enzymatic antioxidant system for the neutralization of excess reactive oxygen species. The purpose of this paper is to evaluate the activity of catalase and peroxidase and proline content in leaves of 14 clones of Salix (7 Romanian and 7 Swedish) grown on fly ash dumps from Isalnita. The obtained data were compared to those determined for control cultures from Radovan. The samples were collected in July and were determined the catalase and peroxidase activity by colorimetric method. The proline content was determined from sulfosalicylic acid extract by colorimetric method with ninhidric acid as reagent using L proline as standard. The results obtained show an increase in peroxidase activity and in proline content (with few exceptions) in plants grown on ash dumps in comparison with the control plantation. The increase in antioxidant enzymes activities suggests a state of oxidative stress, the plants activating a defensive system. Oxidoreductase activity and proline content might be used as biomarkers of tolerance/adaptation of Salix specieson degreded soils.

 

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Published

2018-08-07

Issue

Section

Working Group 3: Fundamentally Disciplines and Environment and Plant Management