OSMOLARITY AND APOPTOSIS IN IVF BOVINE EMBRYOS

Authors

  • Cornelia Milovanov Horia Cernescu Research Unit Faculty of Veterinary Medicine
  • Calin Mircu Horia Cernescu Research Unit Faculty of Veterinary Medicine
  • Mirela Ahmadi Horia Cernescu Research Unit Faculty of Veterinary Medicine
  • Camelia Tulcan Horia Cernescu Research Unit Faculty of Veterinary Medicine
  • Isidora Radulov University of Agricultural Sciences and Veterinary Medicine of Banat "The King of Michael I" of Romania (USAMVB), Faculty of Agriculture
  • Irina Patras Horia Cernescu Research Unit Faculty of Veterinary Medicine
  • Ioan Hutu Horia Cernescu Research Unit Faculty of Veterinary Medicine

Abstract

The aim of this research was to establish the optimal conditions for culture of bovine embryos obtained in vitro and the effects of osmolarity on development and apoptosis. The embryos obtained by in vitro fertilization (IVF) procedure were divided into two different groups. The embryos from the control group were cultured for 7 days in B2-Menezo. The embryos from the experimental group were cultured in medium with NaCl, having variation of concentration and osmolarity. Results showed that there are differences in development stage and apoptosis. Increasing the concentration from 90 to 120mM NaCl generated decreasing of the blastocyst stage (P<0.05), and also the apoptosis was increased in the blastocyst stage. The optimum concentration of NaCl was 90mM and the optimum osmolarity of the medium was between 260-280 mOsmol for the studied IVF bovine embryos, affecting positively the embryos development.

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Published

2015-12-25